• Kit Flexibility – one kit works with a variety of sample types and sizes
• Ultraclean gDNA – top of class A260/A280 and A260/A230 absorbance ratios regardless of sample source
• Improved Design – optimized semi-skirted 96-well plates compatible with centrifugation and most bench top and robotic vacuum manifolds
Multiple Sample Sources, One Kit
The PureLink® Pro 96 Genomic DNA Purification Kit can be used with many different sample types, each with its own optimized protocol outlined in the manual. Examples of sample sources and amounts that can be used with the PureLink® Pro Genomic DNA Purification Kit:
| Source | Maximum Sample Amount |
|---|---|
| Mammalian Cells | 5 x 106 |
| Mammalian Tissue | 25 mg (10 mg for spleen) |
| FFPE Tissue | 1-8 sections of 5-15 µm thick with surface area of 20-50 mm^2 |
| Oragene™ Preserved Saliva | 200 µl |
| Mouse or Rat Tail | 1cm (mouse) 0.5cm (rat) |
| Non-Nucleated Whole Blood (e.g., human, mouse) | 200 µl (single pass) |
| Nucleated Whole Blood (e.g.,bird) | 5-10 µl |
| Blood Spot on Paper | 2-5 punches (2-3mm) |
| Bacterial Cells | 2 × 109 |
| Yeast Cells | 5 × 107 |
Improved Plate Design
The PureLink® Pro 96 Genomic DNA Purification Kit provides optimized, semi-skirted, 96-well plates (Figure 3), for improved performance and results. In addition, the plate nozzles with annular collar prevent cross-contamination of samples and improve drying of the silica membrane to prevent ethanol carryover. Sample processing can be performed manually using a bench top vacuum manifold, by centrifugation (at ~2 x g), or with an automated liquid handling platform. The semi-skirted plate design is compatible with most vacuum manifolds on robotic workstations.
Higher Yields and Purity
The PureLink® Pro 96 Genomic DNA Purification Kit outperforms competitor products in both DNA yield and purity for multiple sample types, including blood (Figure 7). You are also able to get greater yields of higher-purity gDNA (determined by A260/A280 measurements) no matter what your sample type is; from bacteria to tissues to blood to cells (Figures 4-6). The enhanced PureLink® Lysis and Wash Buffers help consistently deliver purified gDNA samples with absorbance reading ratios of ~1.9 for both A260/A280 and A260/A230, indicating no contamination carryover of protein or guanidine (Figure 7).
Contents and Storage
• PureLink® Genomic Binding Plates
• Wash Plates
• Deep Well Plates
• Foil Tape
• Wash Buffers
• Digestion Buffer
• Lysis/Binding Buffer
• Wash Buffers
• Elution Buffer
• Proteinase
• RNase A
• Manual
Store at room temperature. Guaranteed stable for one year when properly stored.
FOR RESEARCH USE ONLY. NOT INTENDED FOR ANY ANIMAL OR HUMAN THERAPEUTIC OR DIAGNOSTIC USE.
