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The PureLink® Genomic DNA Mini Kit enables high-yield, high-purity, genomic DNA (gDNA) extractions from a wide variety of sample types. Use this single kit for genomic DNA purification from blood, tissues, cells, bacteria, swabs, and blood spots, with a familiar silica-based, microcentrifuge spin-column format.
Features of the PureLink® Genomic DNA Mini Kit include:
• Kit Flexibility—one kit works with a variety of sample types and sizes • Ultraclean gDNA—minimal contamination of DNA product means successful downstream application • Improved Design—optimized spin-column design and buffer formulation for better yield and purity
Multiple Sample Sources, One Kit The PureLink® Genomic DNA Mini Kit can be used with many different sample types, each with its own optimized protocol outlined in the manual. Some examples of sample sources and amounts that can be used with the PureLink® Genomic DNA Mini Kit are: • Mammalian cells: 5 × 106 • Mammalian tissue: 25 mg (10 mg spleen) • Formalin-fixed paraffin-embedded (FFPE) tissue: 1–8 sections of 5–15 µm thick (20–50 mm2 surface area) • Oragene® preserved saliva: 4 ml • Rodent tail: 1 cm (mouse), 0.5 cm (rat) • Non-nucleated whole blood: 200 µl (single pass), or 1 ml (multiple pass) • Nucleated whole blood (bird): 5–10 µl • Blood spot on paper: 2–5 punches (2–3 mm) • Bacterial cells: 2 × 109 • Yeast cells: 5 × 107
Optimized Components and Protocols The PureLink® Genomic DNA Mini Kit includes parts and protocols that improve upon the easy and familiar spin-column methods currently in use (Figure 1). Columns are newly configured to bind and release DNA more efficiently (Figure 2). Each sample source uses a specialized lysis procedure and an optimized lysis buffer formulated to enhance proteinase K activity and eliminate protein contamination. A chaotropic salt buffer promotes stable binding of DNA to the column resin, and powerful wash buffers remove all traces of protein and salt. DNA is eluted in a low-salt buffer to allow for pH stabilization of the DNA in storage.
Higher Yields and Purity With PureLink® Genomic DNA Kits you can expect high yields of high-purity gDNA (determined by A260/A280 measurements), no matter what your sample type is - from bacteria to tissues to blood to cultured cells (Figures 4-6). The enhanced PureLink® Lysis and Wash Buffers help to consistently deliver purified gDNA samples with absorbance reading ratios of ~1.9 for both A260/A280 and A260/A230, indicating no detectable contamination carryover of protein or guanidine (Figure 7).
For Research Use Only. Not for use in diagnostic procedures
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