High yield, high quality mRNA using oligo(dT)-based magnetic bead technology
- Pure mRNA in less than 1 hour when starting from total RNA and less than 1.5 hours when isolating directly from cells or tissue
- Three kit formats (Table 1) are available, depending on the amount of starting material
The FastTrack™ MAG mRNA Isolation Kits isolate significantly higher mRNA yield, in less time, than any other mRNA isolation kit (Figure 1). Using oligo(dT)-based magnetic beads, your mRNA yields are as high as 3% of total RNA and more than 1 µg when directly isolated from 1 x 10e6 cells. In minimal time you can go from cell or tissue to highly pure mRNA with minimal contamination from ribosomal RNA or genomic DNA.The FastTrack™ MAG mRNA Isolation Kit elutes mRNA in small volume for concentrated samples that are ready-to-use for many applications, including cDNA library construction, microinjection, in vitro
translation, subtraction, primer extension, RT-PCR, and northern blotting. Some downstream applications may not require elution from the magnetic beads.This kit includes all the necessary reagents for the number of preps listed and requires the use of a magnetic accessory. Please see the Accessories Chapter (Chapter 6) to select the best magnetic accessory for your needs.