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The PureLink® FFPE RNA Isolation Kit provides a simple, reliable, and rapid method to isolate high-quality total RNA from formalin-fixed, paraffin-embedded (FFPE) tissue specimens for retrospective basic and clinical biological studies of gene expression without the use of toxic organic solvents for deparaffinization. • Offers a rapid and environmentally friendly procedure for the extraction of RNA from FFPE tissue specimens • Isolates RNA with maximum yield and minimum degradation
Rapid and Non-Toxic Isolation Procedure The PureLink® FFPE RNA Isolation Kit employs a simple heating procedure for deparaffinization, instead of relying on the use of toxic organic solvents such as xylene. RNA isolation can be completed in 30 - 40 minutes instead of days, as is required for older FFPE isolation methods (Figure 1). The heating procedure for deparaffinization partly restores RNA template structure for cDNA synthesis and improves template quality for reverse transcription.
High Yield, High Quality RNA The PureLink® FFPE RNA Isolation Kit offers higher yields and purity as compared to other commercially available RNA purification systems, allowing for RT-PCR products over 1 kb in length (Figure 2). In addition, the PureLink® FFPE RNA Isolation Kit minimizes genomic DNA contamination of the purified RNA sample and provides an option for DNase digestion.
Purified Total RNA is Suitable for Use with a Variety of Downstream Applications and Products RNA extracted from FFPE specimens is suitable for downstream applications such as reverse transcription, real-time PCR (qPCR), real-time RT-PCR (qRT-PCR), and microarray analysis.
For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.
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