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This cell line is available as dividing cells by requesting a quote for K1291 from firstname.lastname@example.org
GeneBLAzer® MC3R CHO-K1 DA(Division Arrested) cells and MC3R-CRE-bla CHO-K1 cells contain the human Melanocortin subtype 3 receptor (MC3R) stably integrated into the CellSensor® CRE-bla CHO-K1 cell line. CellSensor® CRE-bla CHO-K1 cells (Cat. no.K1129) contain a beta-lactamase reporter gene under control of the Cyclic AMP Response Element (CRE). Division Arrested (DA) cells are available in two configurations: an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384-well plate), and a tube of cells sufficient to analyze 10 x 384-well plates.
DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin-C, and have no apparent toxicity or change in cellular signal transduction. Both MC3R CHO-K1 DA cells and MC3R-CRE-bla CHO-K1 cells are functionally validated for Z’-factor and EC50 concentrations of NDP-α-MSH (Figure 1). In addition, MC3R-CRE-bla CHO-K1 cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time (data available upon request). Additional testing data using alternate stimuli are also available.
The melanocortin-3 receptor (MC3R) is primarily expressed in the hypothalamus, and plays an important role in the regulation of energy homeostasis. MC3R recognizes all of the melanocortin peptides, and notably binds - -MSH with greater affinity than the other melanocortin receptors. MC3R-deficient (MC3R-/-) mice demonstrate increased fat mass, higher feeding efficiency, and reduced lean body mass. To date only one mutation of MC3R has been identified to be associated with human obesity. Functional analysis of this mutation indicated a complete loss of agonist-mediated receptor activation.
GeneBLAzer® MC3R CHO-K1 DA Cells and MC3R-CRE-bla CHO-K1 Cells are shipped on dry ice. Store in liquid nitrogen immediately upon receipt or thaw for immediate use.
Contents: GeneBLAzer® MC3R CHO-K1 DA Assay Kit Each system contains sufficient division arrested (DA) cells & substrate to assay 1 x 384-well plate. (Other materials are required separately; please refer to the protocol). Includes: •MC3R CHO-K1 DA cells •LiveBLAzer™-FRET B/G Loading Kit, 70µg •Solution D, 1ml •2 protocols •Certificate of Analysis GeneBLAzer® MC3R CHO-K1 DA cells Each system contains sufficient division arrested (DA) cells to assay 10 x 384-well plates (LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol). Includes: •MC3R CHO-K1 DA cells •1 protocol •Certificate of Analysis
GeneBLAzer® MC3R-CRE-bla CHO-K1 cells (LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol). Includes: •MC3R-CRE-bla CHO-K1 cells •1 protocol •Certificate of Analysis
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