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The iPrep™ ChargeSwitch® gDNA Tissue Kit allows rapid and efficient purification of DNA from a wide range of tissue samples types. Designed specifically for use with the iPrep™ Purification Instrument, this kit yields larger amounts of highly pure DNA than can be achieved with competing systems (Table 1)-with many tissues, up to 2-fold greater yields are typical. The resulting DNA is also highly pure (Figure 1), and is ideal for use in PCR (Figure 2).
The iPrep™ ChargeSwitch® gDNA Tissue Kit takes advantage of the powerful ChargeSwitch¤ technology (Figure 3), a major advance in DNA purification. This technology is based on a unique, ionizable nucleic acid-binding ligand whose charge can be switched based on the pH of the surrounding medium. Purification is achieved through a simple three-step bind-wash-elute procedure, and avoids the use of guanidine, ethanol, and other troublesome reagents.
Contents and Storage: The iPrep™ ChargeSwitch® gDNA Tissue Kit provides all reagents (supplied in prefilled cartridges), tubes, and tips needed for isolating DNA from tissue samples. Reagent cartridges can be stored at room temperature; store lysis buffer and proteinase K at 4°C.
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