This product contains three boxes of 10 sets/box of iBlot® Transfer Stack, Mini, Nitrocellulose (Cat# IB3010-02)
The iBlot® Dry Blotting System efficiently and reliably transfers proteins from polyacrylamide gels in seven minutes or less without the need for buffers or an external power supply. A self-contained unit (Figure 1), the iBlot® device uses disposable blotting stacks with integrated transfer membranes (nitrocellulose and PVDF), offering the convenience of a bufferless, plug-and-play system. Compared to conventional blotting techniques, the iBlot® system enables higher downstream detection sensitivities.
The iBlot® system is ideal for scientists analyzing proteins through western blotting who want:
• Complete transfer in seven minutes or less
• High detection sensitivity and evenness
• Increased blotting reliability and reproducibility
• Self-contained power supply
• Flexible gel size formats and membrane types
How it Works
Ion reservoirs are incorporated in the gel matrix (transfer stacks) instead of buffer tanks or soaked papers (Figure 2). The high density and availability of ions in the gel matrix enables rapid protein transfers. During blotting, the copper anode does not generate oxygen gas as a result of water electrolysis, which can cause blotting distortion. Conventional electroblotting techniques, including wet, semi-wet, and semidry, use inert electrodes which generate oxygen. With the iBlot® Dry Blotting System, transfer time is reduced by using a shortened distance between electrodes, high field strength, and high currents. Trapped air bubbles, often created during the manual preparation of the blotting sandwich layers, are avoided using a unique de-bubbling design to ensure even and complete transfers.
iBlot® Transfers Save You Time
With the iBlot® system, there’s no need to prepare buffers, pretreat your gel, or clean up after your blotting. The total preparation and run time is less than 10 minutes per blot. Compared to wet and semi-dry transfer techniques, iBlot® transfers save you hours (Table 1).
Quality Protein Transfers
The iBlot® Dry Blotting System evenly and efficiently transfers proteins from gels. With iBlot® transfers, crisp, clear bands remain sharp and straight with high transfer sensitivity (Figure 3).