CD103, Hamster Anti-Mouse, (FITC); FITC conjugated Monoclonal antibody specific to Mouse CD103. This antibody is validated for use in Flow Cytometry. Anti-CD103 recognizes the expressed product of the CD103 gene.
CD103 (Integrin αIEL) is a member of the integrin series of adhesion molecules. This antigen defines a developmentally important subset of T cells, namely mucosal T cells including all IEL (intraepithelial lymphocytes) and ~20% of lamina propria T cells. Expression of CD103 is more restricted outside these mucosal organs, appearing at lower levels on T cell subsets of the lymph node, dendritic epidermis and periphery.1-3 In non-epithelial CD103+ T cells there is a bias toward expression on CD8+.cells. The 2E7 mAb exhibits antigen immunoprecipitation patterns similar if not identical to the rat anti-mouse CD103 mAb M290.1 The 2E7 mAb is reported to have signal-inducing activity in a redirected lysis assay and to co-stimulate IEL and CD8+ lymph node cells in conjunction with anti-TCR in vitro, which runs counter to the usual inhibition of CTL by anti-integrins.1 This co-stimulatory activity is also shared by the mAb M290.4 The property of co-stimulation is not unique to the mouse since the combination of anti-human αIEL plus anti-TCR will produce similar proliferation in human cells, with the exception that CD8+ cells are not stimulated.5
Identification and enumeration of CD103+ cells by flow cytometry
Immunohistochemistry (frozen sections)
In vitro activation
LeFrancois, L., T.A. Bennett, W.L. Havran, and L. Puddington. 1994. Developmental expression of the αIELβ7 integrin on T cell receptor γδ and T cell receptor αβ T cells. Eur. J. Immunol. 24:635.
Lefrancois, L. 1991. Extrathymic differentiation of intraepithelial lymphocytes: generation of a separate and unequal repertoire? Immunol. Today 12(12):436.
Reimann, J. and Rudolphi, A. 1995. Co-expression of CD8α in CD4+ T cell receptor αβ+ T cell migration into the murine small intestine epithelial layer. Eur. J. Immunol. 25:1580.
Roberts, K., and P. Kilshaw. 1993. The mucosal T cell integrin αM290β7 recognizes a ligand on mucosal epithelial cell lines. Eur. J. Immunol. 23:1630.
Cerf-Bensussan, N., Bengue, B., Gagnon, J. and Meo, T. 1992. Eur. J. Immunol. 22:273.