FluoCells® prepared slide #3 mouse kidney section with Alexa Fluor® 488 WGA, Alexa Fluor® 568 phalloidin, DAPI

Cat. No. F-24630

The FluoCells prepared slide #3 is ideal for demonstrating the optical sectioning properties of confocal microscopes. Each slide contains a 16 µm cryostat section of mouse kidney stained with Alexa Fluor 488 wheat germ agglutinin (W-11261), Alexa Fluor 568 phalloidin (A-12380) and DAPI (D-1306). See also FluoCells prepared slide #4 (F-24631).

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	Mouse kidney section. FluoCells® prepared slide #3. FluoCells® prepared slide #3 (Cat. no. F24630) contains a mouse kidney section stained with a combination of fluorescent dyes. Alexa Fluor® 488 wheat germ agglutinin (Cat. no. W11261) is a green-fluorescent lectin that was used to label elements of the glomeruli and convoluted tubules. The filamentous actin prevalent in glomeruli and the brush border were stained with red-orange–fluorescent Alexa Fluor® 568 phalloidin (Cat. no. A12380). Finally, the nuclei were stained with the blue-fluorescent DNA stain DAPI (Cat. no. D1306, D3571, D21490). This pseudocolored image was acquired on a Zeiss confocal microscope located at the Institute of Neuroscience, University of Oregon.
	Mouse kidney section. FluoCells® prepared slide #3. FluoCells® prepared slide #3 (Cat. no. F24630) contains a section of mouse kidney stained with a combination of fluorescent dyes. Alexa Fluor® 488 wheat germ agglutinin (Cat. no. W11261), a green-fluorescent lectin, was used to label elements of the glomeruli and convoluted tubules. The filamentous actin prevalent in glomeruli and the brush border were stained with red-fluorescent Alexa Fluor® 568 phalloidin (Cat. no. A12380). Finally, the nuclei were counterstained with the blue-fluorescent DNA stain DAPI (Cat. no. D1306, D3571, D21490). This image is a composite of three micrographs acquired using filter sets appropriate for fluorescein, tetramethylrhodamine and DAPI.
	Cryostat section of mouse kidney. FluoCells® prepared slide #3, Alexa Fluor® 488 wheat germ agglutinin, Alexa Fluor® 568 phalloidin and DAPI. FluoCells® prepared slide #3 (Cat. no. F24630) containing a 16 µm cryostat section of mouse kidney stained with green-fluorescent Alexa Fluor® 488 wheat germ agglutinin (Cat. no. W11261), red-orange–fluorescent Alexa Fluor® 568 phalloidin (Cat. no. A12380) and blue-fluorescent DAPI (Cat. no. D1306, D3571, D21490). The image represents an optical section obtained by simultaneous two-photon excitation of all three dyes at 797 nm using a Bio-Rad Radiance 2100 multi-photon microscope system. The image was acquired at the 2001 3-D Microscopy of Living Cells Course, University of British Columbia, Vancouver, Canada, by John Jordan, Bio-Rad Laboratories; and Iain Johnson, Molecular Probes, Inc.