Copy and paste this HTML to embed a link in your website
Paste link in email or IM
The EnzChek® Reverse Transcriptase Assay Kit is a convenient, efficient and inexpensive assay for measuring reverse transcriptase activity. The key to this method is the PicoGreen® dsDNA quantitation reagent, which preferentially detects dsDNA or RNA-DNA heteroduplexes over single-stranded nucleic acids or free nucleotides. In the assay, reverse transcriptase activity in a biological sample generates long RNA-DNA heteroduplexes from a mixture of a long poly(A) template, an oligo-dT primer and dTTP. The RNA-DNA heteroduplexes formed are then detected by the PicoGreen reagent. In less than an hour, samples can be read in a fluorometer or microplate reader with filter sets appropriate for fluorescein (FITC). The assay is sensitive, detecting as little as 0.02 units of HIV-1 reverse transcriptase, and has about a 50-fold linear range Because it is much more rapid and less expensive than standard isotopic assay or immunoassays, it is suitable for testing large numbers of biological samples. Its simplicity also makes it useful for automated high-throughput screening of reverse transcriptase inhibitors.
If you continue without changing your settings, we'll assume that you are happy to receive all cookies on Life Technologies websites. However, you can change your cookie settings at any time at the bottom of this page.
To access this page, please close your session and log into the PunchOut session again. You will need to provide an email address on the sign-in page.Please note if you are a punchout to supply center customer, and are viewing this message, your procurement system does not provide the required information to access this tool. Please contact your procurement system administrator for further instructions.