Purification of viral RNA or DNA from cell-free fluids has traditionally been performed using silica plates or silica magnetic-bead separation methods, resulting in eluted, purified nucleic acids in large volumes. The ChargeSwitch® EasyPlex™ Kit eliminates RNA or DNA elution, allowing for complete use of the nucleic acids from 30 µl serum or plasma samples for enzymatic applications including qPCR and qRT-PCR.
With these kits you´ll get:
• Direct amplification of nucleic acids bound to microtiter plate surfaces-no elution step
• Fast processing with minimal handling-from lysis to PCR in under an hour
• Flexible automation-requires no shakers, vacuum manifolds, or centrifugation
The simple process (Figure 1) involves minimal handling, which allows for easy automation and reduces the possibility of false reports related to human error. In addition, the ChargeSwitch® system does not use ethanol for washing RNA or DNA, which means there is less of a chance of PCR inhibition or reduced nucleic acid recovery from inefficient ethanol removal.
Sensitive viral quantitation of serum and plasma using real-time PCR and RT-PCR
The ChargeSwitch® EasyPlex™ system allows for extraction of both viral RNA or DNA for viral analysis protocols ranging from detection to genotyping. Purity of viral RNA or DNA is a critical factor in viral detection analysis since real-time PCR is sensitive to even minute impurities in the sample. To measure both sensitivity and purity of viral RNA and DNA processed using the ChargeSwitch® EasyPlex™ Viral system, we analyzed the recovery of viral nucleic acids from only 10 µl of serum or plasma, using real-time PCR.
Purification using ChargeSwitch® EasyPlex™ is both sensitive and linear down to the limit of 100 RNA virus particles and 1,000 DNA virus particles using the EasyPlex™ Viral system. No difference was observed for plasma versus serum, although plasma is a more proteinacous sample type (Figures 2 and 3). The data indicate that RNA is of high quality and contaminants in the sample are completely removed, as even trace impurities would have impacted the RT-PCR results.
The new revolution:
The ChargeSwitch® EasyPlex™ Viral RNA/DNA Kit represents a significant advance in technology for viral nucleic acid monitoring. ChargeSwitch® technology eliminates ethanol or guanidine in washing the nucleic acids, providing the ultimate in purity of RNA and DNA. The unique ChargeSwitch® coated PCR plate enables concentration of viral nucleic acids in the PCR well for complete use of viral RNA or DNA for downstream applications with minimal handling and without dilution.
Contents and Storage:
The ChargeSwitch® EasyPlex™ Viral RNA/DNA Kit contains ChargeSwitch® Coated Plates for virus, foil tape, Proteinase K, ChargeSwitch® Viral Lysis Buffer, ChargeSwitch® Viral Binding Buffer, and Wash Buffer. The kit is shipped and stored at 4°C.