The CD3, clone UCHT1, Pacific Orange™ dye conjugate is a monoclonal antibody that is reactive to the human CD3 antigen and recognizes the expressed product of the CD3D gene. Our Pacific Orange™ dye is optimally excited by the violet laser, and it is recommended that a 575, 585, or 600 nm band pass filter be used for optimal detection. Pacific Orange™ and Pacific Blue™ dye conjugates can be simultaneously excited at 405 nm and emit at 551 nm and 455 nm, respectively, facilitating two-color analysis.


Specificity for CD3
The UCHT1 monoclonal antibody reacts with human CD3ε, a 20 kDa subunit of the TCR/CD3 complex. Along with the other CD3 subunits γ and δ, the ε chain is required for proper assembly, trafficking, and surface expression of the TCR complex2. CD3 is expressed by developing thymocytes and by all mature T cells. Crosslinking of TCR via immobilized UCHT1 results in T-cell proliferation.

Analyte Specific Reagent
This product is an Analyte Specific Reagent. Analytical and performance characteristics are not established.

Related Links
For the full list of all CD3 antibodies available, please visit the primary antibody search tool.
You can get additional information about violet laser reagents in flow cytometry and use the Spectraviewer to help you plan your experiments. Additional general information about Life Technologies flow cytometry products and support is also available.