MegaX DH10B™ T1^R Electrocomp™ Cells are the highest-efficiency electrocompetent cells available. Improved production processes now guarantee a three-fold greater number of colonies per transformation (>3 x 10¹⁰ cfu/µg of pUC DNA), making the MegaX DH10B™ T1^R cells ideal for highly demanding cloning and library construction. They have the same genotype as the widely used DH10B™ T1^R strain including tonA, which prevents infection by T1 and T5 lytic bacteriophages1 and safeguards your valuable clones and libraries.

MegaX DH10B™ T1^R cells provide highly efficient transformation of methylated DNA, since they lack the E. coli K12 restriction systems (mcrA Δ(mrr-hsdRMS-mcrBC)).^2,3,4 The 80lacZΔM15 marker provides α-complementation of the β-galactosidase gene, allowing blue/white screening on agar plates containing X-gal or Bluo-gal.

Genotype:
F- mcrA Δ(mrr-hsdRMS-mcrBC) 80lacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ^- rpsL nupG tonA

Contents and Storage:
MegaX DH10B™ T1^R Electrocomp™Cells, pUC19 DNA (10 pg/µl), and Recovery Medium are shipped on dry ice. Store at -80°C upon receipt.

References:
1. Killmann, H., Benz, R., and Braun, V. (1996) J. Bacteriol. 178, 6313.
2. Raleigh, E. A. (1988) Nucl. Acids Research 16, 1523.
3. Woodcock, D.M., et al. (1989) Nucl. Acids Research 17, 3469.
4. Blumenthal, R.M. (1989) Focus 11:3, 41.