The Click-iT® Dapoxyl® Glycoprotein Detection Kit provides the second part of a simple and robust two-step technique to identify and characterize glycoproteins by one- or two-dimensional gel electrophoresis. In step two, after the incorporation of an azide moiety into protein glycan structures with either a Click-iT® Metabolic Labeling Reagent or the Click-iT® Enzymatic Labeling System, the azide-modified glycoproteins are detected via the chemoselective ligation or click reaction between an azide and an alkyne. Gels with Dapoxyl® labeled glycoproteins can be subsequently stained with the Multiplexed Proteomics™ technologies, SYPRO® Ruby total protein stain and PRO-Q® Diamond phosphoprotein stain for differential analysis of glycoprotein subclasses, total proteins and total phosphoproteins in the same gel. Dapoxyl-labeled glycoproteins are also compatible with downstream LC-MS/MS and MALDI MS analysis for identification.