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The Click-iT® GlcNAz metabolic glycoprotein labeling reagent provides the first part of a simple and robust two-step technique to identify and characterize intracellular O-GlcNAc glycoproteins. In step one, cultured cells are incubated with the azide-modified glucosamine (GlcNAc). The azido-sugar is incorporated into intracellular O-GlcNAc-containing glycoproteins through the permissive nature of the oligosaccharide biosynthesis pathway. In step two, via the chemoselective ligation or click reaction between an azide and an alkyne, the azido-labeled glycoproteins can then detected with a Click-iT® Glycoprotein Detection kit for gels (TAMRA or Dapoxyl® alkyne) or Western blots (biotin alkyne). The Click-iT® glycoprotein products are compatible with LC-MS⁄MS and Multiplexed Proteomics™ technologies for in-depth analyses of the glycoproteome.
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