CellROX® Orange Reagent is a novel fluorogenic probe for measuring oxidative stress in live cells. This cell-permeant dye is non-fluorescent while in a reduced state and exhibits bright orange fluorescence upon oxidation by reactive oxygen species (ROS), with absorption/emission maxima of ~ 545/565 nm. While this reagent is not formaldehyde-fixable, it can be multiplexed with other live cell-compatible probes such as GFP, CellEvent® Caspase 3/7 Green reagent, Image-iT® DEAD Green, Green and Deep Red MitoTracker® dyes, and NucBlue™ reagent.
CellROX® Orange Reagent is compatible with various platforms, such as traditional fluorescence microscopy, high content screening (HCS), and flow cytometery. This reagent is also compatible with the Tali™ Image-based Cytometer.
In addition, CellROX® Orange Reagent is:
• Compatible with other live cell probes, including GFP, and may be added directly to serum-containing growth media
• Provided as a stable, ready-to-use DMSO solution with a simple protocol compatible with standard workflows in fluorescence microscopy
• Provided as five single-use vials containing enough total reagent for five 96-well plates or 100 coverslips
Oxidative stress results from an imbalance between the production of reactive oxygen species (ROS) and the ability of cells to scavenge them. ROS play an important role in the progression of several diseases, including inflammation, atherosclerosis, aging, and age-related degenerative disorders.
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.