The NativePAGE™ Novex® Bis-Tris Gel System is a pre-cast polyacrylamide mini gel system that provides a sensitive and high-resolution method for analysis of native membrane protein complexes, native soluble proteins, molecular mass estimations, and an assessment of the purity of native proteins. It is based on the blue native polyacrylamide gel electrophoresis (BN PAGE) technique developed by Schagger and von Jagow (1–3). With the NativePAGE™ Novex® Bis-Tris Gel System, you will:
• Simplify resolution of large proteins (15–10,000 kDa)
• Analyze membrane-protein complexes in their native conformations
• Obtain better resolution than with traditional Tris-glycine native electrophoresis
In SDS-PAGE, SDS functions as a charge-shift molecule that denatures proteins by conferring on them a net negative charge and enables proteins to migrate towards the anode. BN PAGE uses Coomassie® G-250 as the charge-shift molecule, which binds to proteins and confers a net negative charge while maintaining the proteins in their nondenatured, native state.
The near neutral pH of the NativePAGE™ Novex® Bis-Tris Gel System provides maximum stability of both the proteins and the gel matrix, resulting in a highly sensitive method for analysis of native membrane-protein complexes and offering superior band resolution over traditional Tris-glycine gel systems.
1. Schagger, H. and von Jagow, G. (1991) Anal. Biochem. 199: 223-231.
2. Schagger, H., Cramer, W.A. and von Jagow, G. (1994) Anal. Biochem. 217: 220-230.
3. Schagger, H. (2001) Meth. Cell Biol. 65: 231-244.