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Recommended for use with the RNAqueous® Kit for purification of total RNA from plant tissues. One bottle of 10 mL Ambion® reagent is provided.
• Removes common plant contaminants such as polyphenolics and polysaccharides • No phenol extraction, alcohol precipitation, or proteinase digestion steps required when used with the RNAqueous® kit
Ambion's Plant RNA Isolation Aid contains polyvinylpyrrolidone (PVP), a high molecular weight polymer that binds to contaminants such as polyphenolics and polysaccharides that are commonly present in plant tissues. Glass fiber filter–based methods of isolation, such as the RNAqueous® Kit, are especially suited for plant RNA isolation because it does not include any alcohol precipitation steps; many common contaminants found in plant tissue are known to co-precipitate with nucleic acid during alcohol precipitation. When used with the RNAqueous® Kit, the procedure consists of disrupting the plant tissue in a guanidinium-based lysis solution to which the Plant RNA Isolation Aid has been added. A brief centrifugation then removes the contaminants. The supernatant is passed through a glass fiber filter which binds RNA. The filter is then washed and the RNA is eluted in a small volume (50 µL or more) of elution solution.
Accessory Product: The Plant RNA Isolation Aid is designed for use with the RNAqueous® Kit (SKU #AM1912) for RNA purification from plant tissues.
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