A novel reagent for transitioning frozen tissue to a state enabling easy extraction of high-quality RNA; frozen tissues are simply submerged in Ambion® RNAlater®-ICE and allowed to thaw overnight at –20°C. It is provided in one bottle containing 25 mL. Once thawed, the tissues can be processed like fresh tissues using standard RNA isolation procedures. No more laborious grinding of frozen tissue to safeguard the RNA.
• Process previously frozen tissues like freshly harvested samples • Thawing tissues in RNAlater®-ICE protects RNA from degradation • No more tissue pulverizing with mortar and pestle and awkward transfer of powder to tube • Easily apportion frozen tissue samples for multiple experiments
Quick Freezing Tissues Preserves RNA Often, tissues that need to be stored prior to RNA isolation are "snap" or "flash" frozen on dry ice or in liquid nitrogen to preserve RNA integrity. RNA in tissue is stable while frozen at –80°C, but thawing the tissue for RNA purification can result in RNA degradation. This is true even if the tissue thaws while in the denaturation solution.
Processing Frozen Tissues is Problematic Frozen tissues are typically ground with a chilled mortar and pestle in order to maintain RNA quality. Liquid nitrogen must be added to the mortar to keep the sample frozen while it is ground. Powdered tissue can also thaw during transfer to a homogenization vessel. This often results in formation of clumps that do not readily disperse in the lysis solution, resulting in RNA degradation and loss.
Process Frozen Tissue Without Jeopardizing RNA Integrity RNAlater®-ICE solves all of these problems. Simply submerge frozen tissue samples in 10 volumes of the solution and store overnight at –20°C (the solution will remain as liquid at these temperatures). As the tissue thaws, RNA integrity is protected. Once treated, tissue can be safely stored at 4°C or even at room temperature (for a limited period of time) and can be further dissected or processed prior to homogenization in a standard RNA isolation lysis buffer. Thus the same frozen tissue sample can be used multiple times for different experiments without compromising RNA integrity.
If you continue without changing your settings, we'll assume that you are happy to receive all cookies on Life Technologies websites. However, you can change your cookie settings at any time at the bottom of this page.
To access this page, please close your session and log into the PunchOut session again. You will need to provide an email address on the sign-in page.Please note if you are a punchout to supply center customer, and are viewing this message, your procurement system does not provide the required information to access this tool. Please contact your procurement system administrator for further instructions.