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Ambion® RNAsecure™ Resuspension Solution (patents pending) is a unique non-enzymatic reagent that will irreversibly inactivate RNases in solution. It is supplied at working concentration for direct resuspension of RNA pellets. Ten tubes containing 1 mL each are provided.
• No post-treatment autoclaving • Safe, easy-to-use reagent inactivates RNases in solution • Inactivation process can be repeated to protect against newly introduced contaminants • Compatible with downstream procedures, such as RT-PCR and in vitro transcription
RNAsecure™ Resuspension Solution is specifically meant for resuspending RNA pellets that may contain trace amounts of ribonuclease (e.g., after total RNA isolation). Note: it cannot reverse RNA degradation that has already occurred, but can prevent further RNA degradation. To use, the RNA pellet is resuspended in the RNAsecure™ Resuspension Solution and heated to 60°C for 10 min. It can be reheated to eliminate any RNase contamination introduced at a later time.
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