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Ambion® RNAsecure™ (patents pending) is a unique non-enzymatic reagent that will irreversibly inactivate RNases in solution. 1 mL supplied. • No post-treatment autoclaving • Safe, easy-to-use reagent inactivates RNases in solutions • Inactivation process can be repeated to protect against newly introduced contaminants • Compatible with downstream procedures, such as RT-PCR and in vitro transcription • Inactivate RNases in Tris and other solutions that cannot be treated with DEPC RNase contamination in reagents used for RNA isolation and analysis can contribute to experimental inconsistency and, at worst, can cause experimental failure. Traditionally, DEPC has been used to treat solutions that come into contact with RNA. DEPC treatment is time-consuming, possibly hazardous, and only eliminates RNases present at the time of DEPC treatment. In addition, some solutions (e.g., primary amine containing compounds such as Tris) cannot be treated with DEPC at all. Finally, DEPC has to be inactivated by autoclaving post-treatment to prevent it from interfering with downstream enzymatic reactions. RNAsecure™ Reagent eliminates these problems. Unlike DEPC, RNAsecure™ can be used on virtually any solution and does not require post-treatment autoclaving. A unique feature of RNAsecure™ is that reheating after the initial treatment will reactivate the RNase-destroying agent to eliminate any new contaminants. RNAsecure™ is supplied as a 25X concentrated stock. It is added to solutions as part of reactions (in vitrotranscription, RT-PCR, etc.), prior to the addition of enzymes, and heated to 60°C for 10 min to inactivate RNase.
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