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Ambion® ANTI-RNase is a protein ribonuclease inhibitor that noncovalently binds and inactivates neutral pancreatic RNase A–type enzymes. It is supplied in one tube containing 10,000 U (15–30 U⁄μL). No inhibition is seen with RNase 1, T1, T2, H, U1, U2, or CL3. ANTI-RNase is distinct from human placental ribonuclease inhibitor in that it has a more robust interaction with RNase and does not release active RNase in the absence of DTT or other reducing agents. ANTI-RNase may be used in any application where placental ribonuclease inhibitor is used. It is ideal for use in invitro transcription and translation, cDNA synthesis PCR, RT-PCR, RNA isolation procedures, and the preparation of RNase-free antibodies. ANTI-RNase is added to reactions to give a final concentration of 1 U⁄μL. Slight inhibition of ANTI-RNase is measured in the presence of DTT (<=20 mM). ANTI-RNase is thoroughly tested for contaminating RNase, exonuclease, and protease activity. Unit Definition:
One unit is the amount of protein required to inhibit the activity of 5 ng RNase A by 50%.
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