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An alternative to bovine DNase I, Ambion® recombinant DNase I (rDNase I), has less residual RNase and other copurified contaminants than other DNases. rDNase is a highly purified product derived from cloned bovine DNase I. The rDNase I is prepared in a host that has RNase levels that are 1 × 107 fold lower than bovine pancreas. Supplied in one tube of 1,000 U. DNase I has historically been prepared from bovine pancreas, one of the richest sources of RNase activity (~1 mg RNase A per gram of tissue). Therefore, it is often hard to obtain DNase I sufficiently free of RNase so that it will not compromise RNA analysis experiments. Safety of biological products is also an emerging issue. Materials derived from bovine sources are of special concern, due to known transmission of bovine spongiform encephalopathy (BSE) to humans via ingestion of cow products. rDNase I now addresses these issues by providing a recombinant replacement for pancreatic bovine DNase I. rDNase I meets or exceeds the performance of native bovine DNase I. Although the recommended usage conditions for DNase I rarely require more than 2 U per reaction, rDNase I passes Ambion®'s stringent RNase contamination quality control assay using 10 times as much enzyme for a incubation that is longer by 32-fold. This result means that rDNase I is qualified RNase-free at 320 times typical usage conditions, creating an unsurpassed margin of safety for every application.
Unit Definition: One unit is defined as the amount of enzyme required to completely degrade 1 µg DNA in 10 min at 37°C.
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