Ambion® LCM staining kit provides superior staining of frozen sections to facilitate identification of target cells for LCM, while preserving RNA quality. Includes sufficient reagents for processing 80 slides.
• Allows identification of target cells without compromising RNA quality or LCM efficiency
• Maintenance of RNA integrity ensures exceptional performance in downstream applications
• Includes a stain that clearly shows cell distribution—ideal for cases where tissue morphology has been disrupted
• Use with RNAqueous®-Micro and MessageAmp™ II Kits for superior gene expression analyses
Laser Capture Microdissection (LCM) is a method for obtaining pure populations of cells from heterogeneous samples. LCM permits the selection and capture of cells, cell aggregates, and discrete morphological structures from thin tissue sections. Captured cells can then be used for nucleic acid studies, including SNP analysis, endpoint and real-time RT-PCR, and mRNA expression profiling. It is often necessary to stain tissue sections so that discrete structures within the tissue can be discerned. To isolate RNA from LCM samples, it is important to use a procedure that minimizes RNA degradation during staining. The LCM Staining Kit provides a novel procedure that avoids exposing the tissue sections to pure water at any step (see Figure). Two different stains are provided with the kit: Cresyl Violet and Acridine Orange.
Easy Identification of Target Cells
The LCM Staining kit has been used with a variety of tissue types including mouse brain, liver, spleen, and kidney and human ovarian tumor, uterine tumor, lymphoma, small intestine, colon, and breast (see Figure 3). The Cresyl Violet stain allows visualization of variations in cell morphology, useful in identifying malignant cells. The Acridine Orange stain offers an advantage when microdissecting single cells, where the visualization of the tissue captured in the cap is not very clear due to the melting of the thermoplastic film. With Acridine Orange, isolated cells will fluoresce on the cap after microdissection, making it easy to assess whether the tissue/cell was effectively microdissected and retrieved (see Figure).