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The Ambion® RNAqueous-Micro Kit is used for phenol-free total RNA isolation from micro-sized samples such as 10-500,000 cultured cells, as few as 10 laser capture microdissected cells, or up to 10 mg tissue using a guanidinium-based lysis/;denaturant and glass fiber filter separation technology. DNA-free™ DNA removal reagents are included in the kit, making the isolated RNA especially suitable for RT-PCR. • Increased sensitivity in downstream assays • Isolates high quality RNA from micro-scale samples • Includes reagents/protocol for RNA recovery from LCM samples The RNAqueous-Micro Kit is optimized for the purification of total RNA from micro-sized samples (10-500,000 cultured cells, as few as 10 microdissected cells, or up to 10 mg tissue). Samples are solubilized in a powerful chaotropic solution, and RNA is purified on a glass fiber filter cartridge specifically designed for very small samples. A simple modification of the protocol allows recovery of both large and small RNA species including tRNAs, 5S rRNA, and microRNAs. The RNA is eluted in a minimal volume of 20 µl. Residual genomic DNA is efficiently removed using the DNA-free system included in the kit. RNAqueous-Micro contains enough reagents for 50 RNA isolations of DNA-free RNA.
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