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The Ambion® RNAqueous®-4PCR Kit is used for phenol-free total RNA isolation from greater than105 cultured cells or more than10 mg tissue using a guanidinium-based lysis/;denaturant and glass fiber filter separation technology. DNA-free™ DNA removal reagents are included in the kit, making the isolated RNA especially suitable for RT-PCR. • DNA-free RNA for RT-PCR • Rapid, phenol-free total RNA isolation • Amplify mRNA 2000X or more for array analysis • Novel reagent for rapid and safe removal of DNase and divalent cations • Recommended for use with more than10 mg tissue or 105 cells The RNAqueous®-4PCR Kit is designed for RNA isolation from small tissue samples (0.5-75 mg) and small- to medium-sized cultured cell samples (~100-107) and used to isolate RNA for applications such as RT-PCR, that would benefit from its robust genomic DNA removal capabilities. When used in conjunction with Ambion® RNAlater® Tissue Collection: RNA Stabilization Solution, most tissue samples can be stored at 4°C and then easily disrupted in standard microfuge tubes using the pestles provided in the kit. There is no need for snap-freezing in liquid nitrogen or grinding in a mortar and pestle.
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