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The Ambion® MEGAclear™-96 Kit is designed for the quick, high-throughput cleanup of any large-scale transcription reaction. MEGAclear™ efficiently separates RNA from unincorporated NTPs, enzymes, and buffer components. Each kit includes sufficient reagents for 96 reactions.
• Purifies transcription reactions in a 96-well format to produce high quality RNA, free of unincorporated NTPs, enzymes, and buffer components • Quick protocol—purify RNA in less than 20 minutes • Can be adapted to robotic platforms
The MEGAclear™-96 Kit offers more consistent results and higher recovery than generic RNA isolation methods that have been adapted for RNA clean-up. The protocol is quick and easy. The sample is mixed with Binding Solution and ethanol, transferred to a 96-well filter plate and washed twice; an elution step releases purified RNA, ready for any application requiring highly pure synthetic or in vitro–transcribed RNA. The MEGAclear-96 Kit can also be integrated with your robotic platform or used with manual methods.
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