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The Ambion® DNA-free™ DNase Treatment & Removal Reagents are designed for the removal of contaminating DNA from RNA samples and for the removal of DNase after treatment. Includes sufficient reagents for 50 reactions.
Features of the Ambion® DNA-free™ DNase Treatment & Removal Reagent set:
• Safely eliminate DNA contamination from RNA samples • No organic extraction or heat inactivation is required • Includes novel reagent to remove DNase • Recombinant DNase I is certified RNase-free
Since no method of RNA isolation can reliably produce RNA that is completely free of contaminating DNA, many researchers choose to treat their RNA samples with DNase I to remove trace levels of DNA, especially prior to RT-PCR experiments. However, the subsequent inactivation and removal of DNase I can be problematic. Although convenient, heat inactivation normally requires that divalent cations present in the DNase Digestion Buffer be chelated or removed prior to heating so that the RNA sample will not undergo enzyme-independent strand scission. Another alternative is phenol extraction followed by alcohol precipitation. This method is time-consuming and shunned by many researchers who either don't want to work with phenol or worry about losing some of their sample. Ambion® DNA-free™ eliminates both of these concerns by supplying a novel DNase Removal Reagent that effectively removes DNase and divalent cations from the reaction mixture. The DNase/cation removal step takes only three minutes. No organic extraction, EDTA addition, or heat inactivation is required. The DNA-free™ DNase Treatment & Removal Reagents comes complete with RNase-free DNase I, an optimized 10X Reaction Buffer, and a novel DNase Removal Reagent.
Accessory Product: TURBO DNA-free™ (SKU# AM1907) is similar to DNA-free™ but includes TURBO™ DNase, an engineered hyperactive DNase that exhibits up to 350% greater catalytic efficiency than wild type DNase. The enzyme also has a 6-fold lower Km for DNA thus enabling effective removal of trace quantities of DNA contamination.
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