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This Ambion® MessageAmp™ II-Bacteria RNA Amplification kit is for amplification of bacterial RNA for highly sensitive, whole-genome expression analysis. The kit employs an in vitro transcription (IVT)–mediated linear amplification method optimized for use with bacterial RNA. The kit includes sufficient reagents for 20 reactions.
• Maximum sensitivity—amplify as little as 10 ng of mRNA or 100 ng total RNA; a 10- to 500-fold reduction in the input RNA needed to probe microarrays • Highest yield of full-length transcripts—includes ArrayScript™ RT which produces up to twice the amount of full-length cDNA as M-MLV, AMV, or SuperScript® II • Stringently reliable data—reactions are highly reproducible, with Pearson's Correlation Coefficients >0.995
This kit is the only commercially available kit for amplification of prokaryotic mRNA. The kit is compatible with any bacterial species and input RNA prepared from multiple sources, such as total RNA, bacterial mRNA or RNA. aRNA generated by the MessageAmp™ II-Bacteria Kit can be labeled by either inclusion of a labeled nucleotide in the IVT reaction, or by reverse transcription of the aRNA in a reaction containing a labeled nucleotide. The MessageAmp™ II-Bacteria Kit can thus be used to prepare both 33P-labeled sense strand cDNA (for nylon PCR arrays) and biotin- or fluorescent dye–labeled aRNA for glass slide microarrays or Affymetrix GeneChip® arrays.
Accessory Products Bacterial mRNA can be enriched from total RNA using the MICROBExpress™ Bacterial mRNA Enrichment Kit (SKU#AM1905).Bacterial RNA can be separated from host cell mixtures with the MICROBEnrich™ Kit (SKU#AM1901).
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