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The Ambion® QuantumRNA™ 18S Internal Standard with Competimer™ technology uses specially modified Competimer™s of the same sequence as the normal 18S primers that cannot be extended. By adjusting the ratio of 18S Competimer™s to normal 18S mRNA primers, the signal for 18S mRNA can be attenuated to the level of very rare messages. They are used for quantitative end-point RT-PCR and include sufficient reagents for 100 reactions. Because of its invariant expression across tissues and treatments, 18S ribosomal RNA is an ideal internal control for quantitative RNA analysis. The Universal 18S Internal Standards function across the broadest range of eukaryotes, including animals, plants, and many protozoa and produce a 315 bp PCR product.
Accessory Products: The QuantumRNA™ Classic 18S Internal Standard (SKU# AM1716) produce a different 489 bp PCR product, and the QuantumRNA™ Classic II 18S Internal Standard (SKU# AM1717) produce a 324 bp PCR product.
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