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The Ambion® RETROscript® First Strand Synthesis Kit includes reagents for reverse transcription of RNA into double-stranded cDNA products. Includes sufficient reagents for 40 reactions.
• Amplify specific RNA targets from complex mixtures (i.e. total cellular RNA) • Both random decamers and oligo(dT)18 primers provided • Perform either one- or two-step RT-PCR
The RETROscript® First Strand Synthesis Kit provides reagents for reverse transcription of cDNA from RNA templates. When used with SuperTaq™ thermostable DNA polymerase (available separately), the cDNA templates are efficiently amplified into double-stranded DNA products. The Molony-Murine Leukemia Virus (M-MLV) Reverse Transcriptase is used in the first step to extend a primer hybridized to an RNA sample. M-MLV has higher stability and lower intrinsic RNase H activity than AMV Reverse Transcriptase. Both random decamers and oligo dT are provided in the RETROscript® Kit. The kit also includes two alternative buffers for first strand synthesis. Protocols are provided for performing the reverse transcription and PCR amplification reactions sequentially (two-step) or as a single reaction.
Accessory Products: SuperTaq™ Polymerase (SKU #AM2054 and #AM2056) and SuperTaq™ Plus Polymerase (SKU #AM2054 and #AM2056) are recommended for use with the RETROscript® Kit. RETROscript® Kit components that are available separately include dNTPS for cDNA Probe Synthesis (SKU #AM8200), Oligo (dT) Primer (SKU #AM5730G), and Random Decamers (SKU #AM5722G). Also available are the T7-Oligo (dT)24 Promoter Primer (SKU #AM5710) and the T7-Oligo (dT)24 Promoter Primer, Anchored (SKU #AM5712).
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