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A synthetic miRNA inhibitor for miRNA functional analysis and target site validation. 5 nmol is supplied.
• Ambion® Pre-designed inhibitors for miRNAs listed in miRBase—custom design available • Purified, ready-to-transfect molecule that specifically inhibits endogenous miRNA • Single-stranded, modified RNA design results in tight binding and efficient inhibition
Anti-miR™ miRNA Inhibitors are chemically modified, single-stranded nucleic acids designed to specifically bind to and inhibit endogenous microRNA (miRNA) molecules. These ready-to-use inhibitors can be introduced into cells using transfection or electroporation parameters similar to those used for siRNAs. They enable detailed studies of miRNA biological effects via miRNA loss-of-function experiments, such as: • Screening for miRNAs that regulate the expression of a gene • miRNA target site identification and validation • Screening for miRNAs that affect a cellular process
To select your miRNA sequence and order, please visit the GeneAssist™ miRNA Workflow Builder.
Accessory Products: miRNA-related cellular functions can be further investigated using Pre-miR™ miRNA Precursor Molecules, which enable gain-of-function experiments through the increase of miRNA activity.
The Pre-miR™ miRNA Starter Kit (SKU #AM1540) can be used to optimize transfection of Pre-miR miRNA Precursors. Anti-miR™ let-7c miRNA Inhibitor Positive Control (SKU #43924310) provides a convenient, validated positive control for experiments using Anti-miR miRNA Inhibitors;Anti-miR™ miRNA Inhibitor Negative Control #1 (SKU #AM17010) is a random sequence that has been validated to produce no identifiable effects on known miRNA function. Cy™3– and FAM™ dye–labeled Anti-miR Negative Controls (SKU #AM17011 and #AM17012, respectively) are also available.
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