Perfect for researchers new to RNAi, this Ambion® kit contains all of the reagents, protocols, and instructions necessary to get underway with siRNA transfection and ensure successful gene knockdown experiments.
• The perfect choice for those beginning siRNA experiments
• Includes transfection agent, siRNAs, and three different systems for assessing knockdown
• For use with human, mouse, and rat cells
The Silencer® siRNA Starter Kit includes a high-quality, lipid-based transfection agent, siPORT™ NeoFX™, that effectively delivers siRNAs to a broad range of cell lines. It can be used for reverse transfection, a streamlined protocol that eliminates an entire day from the RNAi experimental procedure; it can also be used with standard transfection protocols. This reagent is not sensitive to serum and efficiently transfects low concentrations of siRNA. The kit also includes a well-characterized GAPDH siRNA, which has been shown to strongly down-regulate GADPH in human, mouse, and rat cells, as well as a nontargeting negative control siRNA, Silencer® Negative Control #1 siRNA. Because GAPDH is ubiquitously expressed and is easy to detect at both the mRNA and protein level, it is an ideal target for a positive control siRNA.
Three Detection Systems
The kit includes three different systems for monitoring GAPDH siRNA-induced knockdown. An anti-GAPDH antibody is provided to assess GAPDH protein levels by western blot or immunofluorescence. The KDalert™ GAPDH Assay is also provided to quickly measure GAPDH enzymatic activity. This assay can also be used to assess cell viability. Finally, RT-PCR primers are included, for real-time quantitative RT-PCR detection of GAPDH mRNA using SYBR® Green. These reagents can also be used to transfect miRNAs, such as the Ambion® Pre-miR™ miRNA Precursor Molecules.