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The Ambion® MEGAscript® RNAi Kit is for the synthesis of large mass amounts of dsRNA for RNAi experiments in nonmammalian systems. The MEGAscript RNAi Kit uses Ambion's patented high-yield transcription technology and includes reagents for transcription, nuclease digestion, and clean up. The kit includes sufficient reagents for 20 reactions.
Synthesize Large Amounts of dsRNA: The ability to generate large amounts of clean, double-stranded RNA is essential for the success of RNAi experiments. The MEGAscript RNAi Kit allows RNAi researchers to synthesize 50 to 100 µg or more of double-stranded RNA in a single transcription reaction. The yield is typically 10–50 times the amount of RNA produced by conventional transcription reactions. DNA of either PCR or plasmid origin can be used as a template for the transcription reaction. The sense and antisense strands can be synthesized from a PCR-generated DNA template containing T7 RNA Polymerase promoters on both ends of the template, eliminating the need for a separate annealing step.
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