Vana™ PARIS Kit is for the purification of both native protein and RNA (including small RNA) from the same sample. Each kit contains sufficient reagents and consumables for either 40 isolations of protein and total RNA (including small RNAs), or 20 isolation of protein and separate large and small RNA fractions.
• Isolate small RNA-containing total RNA and native protein from the same sample
• Start with 100 – 107
cultured cells or up to 100 mg of many mammalian tissues
• Enrich for small RNA <200 nt to increase sensitivity in downstream analyses
• Simple 30-minute procedure
• Ideal for correlating mRNA, miRNA or siRNA, and protein levelsHow the Kit Works
Vana™ PARIS Kit procedure begins with homogenization of samples with a special Cell Disruption Buffer that includes nonionic detergent. Protein remains intact, so a portion of the lysate can be used directly for common protein analysis applications. The remainder of the lysate is used for RNA isolation, using a procedure that combines the advantages of organic and solid-phase extraction, while avoiding the disadvantages of both.Accessory Products:
For tissues that have very high levels of ribonucleases, such as pancreas or spleen, the mirVana™ miRNA Isolation Kit
is recommended, although it not designed to recover protein and small RNA from the same sample. (The kit may also be purchased without phenol
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.