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Novel transcription reaction conditions and Ambion's patented, high-yield technology allow the synthesis of large mass amounts of RNA produced from small templates (<300 bases). Each Ambion® kit includes sufficient reagents for 25 reactions.
• Efficiently transcribes small templates (<300 bases) with consistently high yields • Designed for use with plasmid, synthetic oligonucleotide, or PCR product templates
Short transcripts require many more transcription initiation events to synthesize a given mass of product than do longer transcripts. Since the initiation step is the rate-limiting step in transcription reactions, the yield of product from short templates is often low. The MEGAshortscript™ High Yield Transcription Kit has been designed to maximize the yield of short RNA transcripts from plasmid, oligonucleotide, and PCR templates. MEGAshortscript™ Kits are only optimized for use with the T7 polymerase included in the kit. Using a different polymerase other than that which is supplied in the kit may result in low yields.
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