This purified Mouse anti-ErbB-2 Monoclonal Antibody is specific to human, rat, and mouse ErbB2 (HER2). Over-expression of c-erbB-2 is observed in 15–40% of primary human breast tumors and correlates with poor prognosis in node positive breast cancer. Over-expression of c-erbB-2 is also observed in ovarian, gastric, salivary, and non-small cell lung carcinomas. Validated applications for mouse anti-ErbB-2 monoclonal antibody are immunoprecipitation, immunohistochemistry, flow cytometry, and Western blotting.

Antibody Attributes:

• Applications: Validated applications for Mouse anti-ErbB-2 monoclonal antibody are immunoprecipitation, immunohistochemistry, flow cytometry and Western blotting.
• Host Species and Isotype: The host species and isotype of the ErbB-2 monoclonal antibody is mouse IgG1.
• Clone ID of Monoclonal Antibody (mAb): The ErbB-2 monoclonal antibody clone is e2-4001.
• Reactivity: This ErbB-2 monoclonal antibody is specific to human, rat, and mouse ErbB2 (HER2).
• Product Size: ErbB-2 Monoclonal Antibody, Mouse (clone e2-4001) is available in a 100 µg pack size.

This antibody recognizes c-erbB-2, a receptor protein tyrosine kinase with Mr=185 kDa, which is also known as p185HER-2. c-erbB-2 is the cellular homolog of the neu oncogene. c-erbB-2 is comprised of an extracellular ligand binding domain, a single transmembrane domain, a tyrosine kinase domain, and a C terminal region which mediates signaling. c-erbB-2 forms heterodimers with other members of the EGF receptor family, such as c-erbB-1. In response to ligand binding, c-erbB-2 is autophosphorylated on five tyrosine residues at its C terminus, producing docking sites for SH2-domain containing proteins such as phospholipase Cγ, PI3-K, Src, CHK, and others.

Over-expression of c-erbB-2 is observed in 15–40% of primary human breast tumors and correlates with poor prognosis in node positive breast cancer. Over-expression of c-erbB-2 is also observed in ovarian, gastric, salivary, and non-small cell lung carcinomas.