This rabbit anti-FAK polyclonal antibody is specific to human, mouse, rat, Xenopus and Chicken FAK. FAK polyclonal antibody recognizes the expressed product of the PTK2 gene. This antibody recognizes a protein of 125 kDa, identified as focal adhesion kinase (FAK). FAK is a widely expressed non-receptor protein tyrosine kinase discovered as a substrate for Src and as a key element of integrin signaling. Validated applications for FAK polyclonal antibody are immunofluorescence, Western blotting, and immunoprecipitation.

Antibody Attributes:

• Applications: Validated applications for FAK polyclonal antibody are immunofluorescence, Western blotting, and immunoprecipitation.
• Host Species and Isotype: The host species and isotype of this FAK polyclonal antibody is rabbit IgG.
• Reactivity: FAK polyclonal antibody detects human, mouse, rat, Xenopus, and chicken FAK.
• Product Size: FAK polyclonal antibody is available in a 500 µg pack size.

This antibody recognizes a protein of 125 kDa, identified as focal adhesion kinase (FAK). FAK is a widely expressed non-receptor protein tyrosine kinase discovered as a substrate for Src and as a key element of integrin signaling. Regulation of FAK includes phosphorylation at multiple tyrosine and serine residues. Increased FAK tyrosine phosphorylation occurs upon integrin engagement with fibronectin. Phosphorylation of tyrosine generally is associated with positive regulation and growth promotion; however, dephosphorylation at these sites occurs as cells begin to enter the M-phase of the cell cycle. In contrast, serine phosphorylation either remains high or is increased as cells enter mitosis. FAK plays a central role in cell spreading, differentiation, migration, cell death and acceleration of the G1 to S phase transition of the cell cycle. FAK binds multiple signaling proteins including p130Cas, Graf, Grb2, Src family SH2 domains, and the p85 subunit of PI3-kinase. The epitope is localized in the C-terminal region (amino acid residues 853-1052).