This rabbit anti-rat NMDA Receptor Subunit 2A polyclonal antibody is specific to mouse, rat and human NMDA receptor,subunit 2A. N-methyl-D-aspartate (NMDA) receptors constitute cation channels of the central nervous system that are gated by the excitatory neurotransmitter L-glutamate. Validated applications for NMDA Receptor Subunit 2A polyclonal antibody are Western blotting and immunohistochemistry.
Applications: validated for Western blotting and immunohistochemistry
Host species and isotype: rabbit IgG
Reactivity: human, mouse, and rat NMDA receptor, subunit 2A
Product size: 10 µg pack size
N-methyl-D-aspartate (NMDA) receptors constitute cation channels of the central nervous system that are gated by the excitatory neurotransmitter L-glutamate. Activation of NMDA receptors is essential for inducing long-term potentiation (LTP), a form of activity-dependent synaptic plasticity that is implicated in the learning process in animal behavioral models.3 The biophysical properties of NMDA receptor channels contributing to LTP include Ca2+ permeability, voltage-dependent Mg2+ block and slow-gating kinetics. NMDA receptor channel activities play a role in neuronal development and in disorders such as epilepsy and ischemic neuronal cell death. As targets for ethanol, NMDA receptors may also function in the pathology of alcoholism.
in vitro reconstitution experiments with cloned NMDA receptor type 1 subunit and any one of four type 2 subunits, 2A, 2B, 2C and 2D, revealed that the physiological and harmacological properties of the heteromeric NMDA receptor appear to be imparted by the particular type 2 subunit. Subunits 2A and 2B are detected predominantly in the hippocampus and cortex, whereas 2C is found mainly in the cerebellum. Thus, cellular expression profiles of the NMDA receptor subunits may contribute to the biophysical properties of NMDA receptors in specific central neurons.
For neurobiologists, Molecular Probes provides affinity-purified rabbit polyclonal antibodies to NMDA receptor subunits 2A, 2B and 2C. The anti–NMDA receptor 2A antibody (anti- NR2A) was raised against a fusion protein containing amino acid residues 1253–1391 of the C-terminal region of rat brain subunit 2A; this antibody is specific for the ~180,000-dalton 2A subunit from rat, mouse or human. The anti-NR2B antibody was raised against a fusion protein containing amino acid residues 984–1104 of the C-terminal region of rat brain subunit 2B; this antibody is specific for the ~180,000-dalton 2B subunit from rat, mouse or human. The anti-NR2C antibody was raised against a fusion protein containing amino acid residues 25–130 of the Nterminal region of rat brain 2C subunit; this antibody recognizes the 140,000-dalton 2C subunit, as well as the 180,000-dalton 2A and 2B subunits from rat, mouse or human. In Western analysis, this cross-reacting activity can be tolerated because of the easily distinguished molecular weights. Anti-NR2C antibody can also be used for immunoprecipitation from extracted tissues, such as cerebellum, where the 2C subunit is abundant.
The antibodies have been fractionated from sera by column chromatography in which rat NMDA receptor fusion proteins were bound to a matrix. The affinity-purified anti–NMDA receptor antibodies to subunits 2A (A-6473), 2B (A-6474) and 2C (A-6475) are suitable for immunohistochemistry, Western blot analysis, enzyme-linked immunosorbent assay (ELISA) and immunoprecipitation.