This anti-Alexa Fluor® 405/Cascade Blue®, rabbit IgG antibody was developed to overcome certain limitations of the Alexa Fluor® 405 and Cascade Blue® dyes, which are employed as polar tracers for neuronal cell labeling. Even though the cell soma of a dye-filled neuron may be brightly stained, its finer processes can sometimes be faint and may fade rapidly or be obscured by the more intensely stained portions of the neuron. By using this anti-dye antibody in conjunction with standard enzyme-mediated immunohistochemical methods one can develop a more permanent, fade-free signal for light microscopy.