The Amplex® ELISA Development Kit for Mouse IgG provides a comprehensive set of components for creating an absorbance or fluorescence-based ELISA using a mouse primary antibody. The assay is based on Amplex® UltraRed reagent, a fluorogenic or chromogenic for horseradish peroxidase (HRP) that reacts with H2O2 in a 1:1 stoichiometric ratio to produce Amplex®UltroxRed product, a brightly fluorescent and strongly absorbing reaction product (excitation/emission maxima ~568/581 nm). When used in fluorescence-based assay, Amplex® UltraRed reagent delivers better sensitivity and a broader assay range than colorimetric reagents.