Like our Alexa Fluor 488 dye, the green-fluorescent Alexa Fluor 514 succinimidyl ester is far superior to fluorescein in both brightness and photostability, and it can be detected using standard fluorescein, Oregon Green dye or Alexa Fluor 488 dye filter sets. By using instruments that collect spectral distribution information, such as the Zeiss LSM 510 META and the Leica TCS SP2, the signal from the Alexa Fluor 514 dye can be distinguished from both the Alexa Fluor 488 and Alexa Fluor 500 dyes.