This green-fluorescent mouse anti-GFAP monoclonal antibody Alexa Fluor® 488 conjugate can be used to aid in the identification of cells of glial lineage. The 50 kDa type III intermediate filament protein glial fibrillary acidic protein (GFAP) is a major structural component of astrocytes and some ependymal cells. In the peripheral nervous system, the GFAP monoclonal antibody stains Schwann cells, satellite cells and enteric glial cells. No positive staining is observed in skin, connective tissue, adipose, lymphatic, muscle or gastrointestinal tissues; nor is their staining in liver, pancreas, kidney, ureter, or bladder tissues. This Mouse anti-GFAP monoclonal antibody does not cross-react with vimentin. Validated Applications for the GFAP Monoclonal Antibody, Mouse, Alexa Fluor® 488 Conjugate are immunocytochemistry and immunofluorescence.

Antibody Attributes:
• Applications: Validated applications for this GFAP monoclonal antibody Alexa Fluor® 488 conjugate are immunocytochemistry and immunofluorescence.
• Host Species and Isotype: The host species and isotype of this GFAP monoclonal antibody is mouse IgG1κ.
• Clone ID of Monoclonal Antibody (mAb): The GFAP monoclonal antibody clone is 131-17719.
• Reactivity: The GFAP monoclonal antibody detects human GFAP. It does not cross-react with vimentin.
• Product Size: GFAP monoclonal antibody, mouse (clone 131-17719), Alexa Fluor® 488 conjugate is available in a 50 µl pack size.

The 50 kDa type III intermediate filament protein glial fibrillary acidic protein (GFAP) is a major structural component of astrocytes and some ependymal cells. GFAP associates with the calcium binding protein annexin II-p2 and S-100.Association with these proteins together with phosphorylation regulates GFAP polymerization. Astroycytes respond to brain injury by proliferation (astrogliosis), and one of the first events to occur during astrocyte proliferation is increased GFAP expression. Molecular Probes' anti-GFAP monoclonal antibody, clone 131-15019 (IgG1κ), can be used to aid in the identification of cells of glial lineage. Interestingly, antibodies to GFAP have been detected in individuals with dementia, and tumors of glial origin contain high counts of GFAP. In the central nervous system, anti-GFAP stains astrocytes and ependymal cells. In the peripheral nervous system, the antibody stains Schwann cells, satellite cells and enteric glial cells. No positive staining is observed in skin, connective tissue, adipose, lymphatic, muscle or gastrointestinal tissues; nor is their staining in liver, pancreas, kidney, ureter or bladder tissues. Molecular Probes' anti-GFAP does not cross react with vimentin, which is frequently co-expressed in some astrocytes and Bergmann glia cells, and also in gliomas and other glial cell-derived tumors. Molecular Probes also offers two Alexa Fluor® dye-labeled anti-GFAP antibodies to aid in multi-labeling experiments.