The biotin-XX conjugate of anti-HuC/HuD neuronal protein mouse monoclonal antibody recognizes the Elav family members HuC, HuD and Hel-N1, which are all neuronal proteins. Anti-HuC/D monoclonal 16A11 does not recognize HuR, another Elav family member that is present in all proliferating cells. Mouse anti-HuC/HuD neuronal protein antibody, biotin-XX conjugate can be used in applications such as western blotting, immunocytochemistry and immunofluorescence.

Antibody Specifications:

• Applications: The validated applications for this antibody are western blotting, immunohistochemistry, and immunofluorescence.
• Host Species and Isotype: The host species and isotype of the antibody is mouse IgG2b.
• Clone ID of Monoclonal Antibody (mAb): The monoclonal antibody clone is 16A11.
• Reactivity: Reacts with human HuC/D
• Product Size: Mouse HuC/HuD Neuronal Protein Monoclonal Antibody, Biotinn-XX is available in a 100 µg pack size.

Anti-Hu antibodies were originally isolated from a patient with paraneoplastic encephalomyelitis, a rare condition of selective neural tissue injury associated with small cell lung carcinoma. The antibodies bind specifically to antigens present exclusively in neuronal cells and are thus useful as markers of neuronal cells in tissue. The Hu antigen is an RNA-binding protein of the embryonic lethal abnormal visual (Elav) family. Molecular Probes® anti-human neuronal protein HuC/HuD, mouse monoclonal antibody 16A11 (isotype, IgG2bκ), recognizes the Elav family members HuC, HuD and Hel-N1 which are all neuronal proteins. Anti-HuC/D monoclonal 16A11 does not recognize HuR, another Elav family member that is present in all proliferating cells. The antibody has been shown to specifically label neuronal cells in zebrafish, chick, canaries and humans and is likely to label neuronal cells in most vertebrate species. Labeling is visible early in development, at about the time that the neurons leave the mitotic cycle. The unlabeled anti-HuC/D mouse monoclonal antibody 16A11 is generally used in conjuction with a secondary antibody, such as labeled goat anti-mouse IgG. Molecular Probes® also provides biotin-XX, Alexa Fluor® 488 and Alexa Fluor® 594 conjugates of anti-HuC/D monoclonal 16A11. The biotin-XX labeled antibody can be detected by using fluorescently labeled avidin, streptavidin or NeutrAvidin™ conjugates. The biotin-XX- and the Alexa Fluor-labeled conjugates of anti-HuC/D are preferred for staining mouse tissue, to avoid the possible background staining from the use of a secondary anti-mouse IgG antibody.