Essential 6™ Medium is a feeder-free and xeno-free medium that supports the reprogramming of somatic cells and differentiation of human pluripotent stem cells (PSCs). The formulation is based on a medium originally developed by Guokai Chen et al. (1) in the laboratory of James Thomson and published as "E8." Essential 6™ Medium has been proven to support somatic cell reprogramming using a variety of methods, including episomal vectors (2) and CytoTune™ (Sendai virus). In addition, Essential 6™ Medium can be used as a base for the formulation of custom media for the culture of PSCs.
Essential 6™ Medium enables:
• Reprogramming—does not contain TGFΒ, which has a negative effect on reprogramming efficiency
• Differentiation—does not contain bFGF, which inhibits embryoid body formation and differentiation
• Flexibility—provides a flexible format where the levels of TGFΒ and bFGF can be adjusted and additional components can be added to match a given application
Essential 6™ Medium allows for defined and feeder-free reprogramming when used with bFGF. The formulation supports somatic cell reprogramming using a variety of methods, including episomal vectors (2) and CytoTune™ (Sendai virus), and is optimized to help ensure maximum cell health and pluripotency with minimal variability.
Essential 6™ Medium is a reliable and robust medium for the formation of embryoid bodies. Unlike other media used in PSC culture, Essential 6™ Medium does not contain bFGF or TGFβ, which inhibits embryoid body formation and differentiation.
Essential 6™ Medium is xeno-free and contains only the essential components needed for stem cell culture, minus bFGF and TGFΒ. This provides a basal medium that will ensure maximum cell health and pluripotency, while allowing levels of TGFΒ and bFGF to be adjusted and additional components to be added to match a given application.
Commercialized in partnership with Cellular Dynamics International.
(1) Chen G, Gulbranson DR, Hou Z, Bolin JM, Ruotti V, Probasco MD, Smuga-Otto K, Howden SE, Diol NR, Propson NE, Wagner R, Lee GO, Antosiewicz-Bourget J, Teng JM, Thomson JA. Nat Methods 2011 Apr 10.
(2) Yu J, Hu K, Smuga-Otto K, Tian S, Stewart R, Slukvin II, Thomson J. (2009) Science 324:797-801.