|Description:||CD45.1/Ly-5.1, Mouse IgG2a monoclonal|
|Validated Application:||Flow Cytometry|
|Ex/Em and laser configuration:||488/519 nm, blue laser|
|Leukocyte Workshop Status:||NA|
The CD45 antigen is an essential regulator of leukocyte activation and development, and is now known to be a protein tyrosine phosphatase; for example, CD45 is able to dephosphorylate the Src family of protein kinases. This activity is now known to be required for signal transduction induced by T and B cell antigen receptor engagement, cytokine signaling, and Fc receptor stimulation. The functional state of CD45 also has a pronounced effect on lymphocyte development. CD45 exists in multiple forms, such as in restricted variants (CD45R; see companion products 1660, 1665, 1675, 1685 and 1820) and as the allelic forms CD45.1/Ly-5.1 and CD45.2/Ly-5.2. The former arises as a result of alternative splicing of different exons. The specificity of antibodies to CD45.1 and CD45.2 has been exploited in studies where resolution of donor and recipient cells is essential, such as in gene therapy and bone marrow transplantation. The monoclonal antibody A20 recognizes all mouse leukocytes expressing the CD45.1 allotype.
For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.