|Description:||Ly-6A/E (Sca-1), Rat (Lewis) IgG2a monoclonal|
|Validated Application:||Flow Cytometry|
|Ex/Em and laser configuration:||488/519 nm, blue laser|
|Leukocyte Workshop Status:||NA|
Ly-6A/E, also known as Sca-1, is a member of the Ly-6 multigene family of type-V glycophosphatidylinositol-anchored cell surface proteins. It is expressed on multipotent hematopoietic stem cells in bone marrow of mice with both the Ly-6.1 and Ly-6.2 allotypes. In the periphery, Ly-6A/E exhibits a pattern of expression which is based on differences between the two allotypes. Ly-6.1 strains (e.g., A, BALB/c, CBA, C3H/He, DBA/1, NZB) have few Ly-6A/E+ resting peripheral lymphocytes, whereas Ly-6.2 strains (e.g., AKR, C57BL, C57BR, C57L, DBA/2, PL, SJL, SWR, 129) have relatively large numbers of Ly-6A/E+ lymphocytes. The expression of Ly-6A/E is dramatically upregulated in all strains upon cellular activation. Studies with MAb D7 suggest that Ly-6A/E may be involved in B- and T-lymphocyte responses, and it appears that the antigen may be required for T-cell receptor-mediated T-cell activation.
For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.