The CD3ε (clone 145-2C11) R-PE (phycoerythrin) conjugate is a monoclonal, Armenian hamster antibody that recognizes an epitope on the 25kD ε-chain of the CD3/TCR complex. R-PE is a stable and highly soluble phycobiliprotein that provides maximal absorbance and fluorescence without susceptibility to internal or external fluorescence quenching, thereby affording exceptional quantum yields and molar extinction coefficients. The use of PE in antibody conjugates provides probes with greatly enhanced detectability.

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CD3ε
CD3ε is a member of the immunoglobulin superfamily of cell surface receptors, and is comprised of five invariable chains ranging in size from 16-28 kDa and is closely associated with the T-cell antigen receptor (TCR). It is expressed on all T cells of all mouse strains. CD3 plays a major role in signaling during antigen recognition, leading to T-cell activation. The 145-2C11 monoclonal antibody recognizes an epitope on the 25kD ε-chain of the CD3/TCR complex. In the presence of Fc receptor-bearing accessory cells, soluble 145-2C11 can activate primed and naive T cells in vitro. 145-2C11 can also induce redirected lysis of specific target cells by CTL clones, and can block lysis of specific target cells by antigen-specific CTL’s. Immobilized 145-2C11 can activate both normal T lymphocytes and cloned T-cell lines. Under certain conditions, T-cell activation by 145-2C11 may result in apoptotic cell death.

For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.