Vitronectin (VTN-N) is a recombinant human protein that provides a defined surface for feeder-free culture of human pluripotent stem cells (PSCs). When used with Essential 8™ Medium, vitronectin (VTN-N) has been proven to maintain pluripotency and normal growth characteristics in multiple PSC lines. In addition, vitronectin (VTN-N) has been shown to support PSC growth for >50 passages without any signs of karyotypic abnormalities and maintain the ability of PSCs to differentiate into all three germ line lineages. As published by Guokai Chen et al.(1) in the laboratory of James Thomson, the VTN-N variant of vitronectin supports human pluripotent stem cell attachment and survival better than wild-type vitronectin when used in conjunction with Essential 8™ Medium.

Note: The prototype status of this product means that the product is currently undergoing real time stability studies in compliance with cGMP regulations.

Vitronectin (VTN-N) is:
• Optimized: designed in the laboratory of James Thomson for use with Essential 8™ Medium
• Defined: for reduced variability in your PSC cultures
• Cost effective: for economical and scalable PSC culture

Optimized and Defined Surface
Unlike standard basement membrane extracts (BMEs), vitronectin (VTN-N) was designed in the laboratory of James Thomson for use with Essential 8™ Medium and has been shown to support human pluripotent stem cell attachment and survival better than wild-type vitronectin. Vitronectin (VTN-N) provides an optimal environment for feeder-free pluripotent stem cell culture.

Reduce Variability
Vitronectin (VTN -N) is a defined, recombinant human protein which reduces variability in your PSC cultures compared to human plasma-derived vitronectin and standard basement membrane extracts (BMEs).

Cost Effective
When used with Essential 8™ Medium, vitronectin (VTN-N) provides a cost effective, defined system for feeder-free culture of human pluripotent stem cells (PSCs).

Commercialized in Partnership with Cellular Dynamics International.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Reference:
Chemically defined conditions for human iPSC derivation and culture; Chen G, Gulbranson DR, Hou Z, Bolin JM, Ruotti V, Probasco MD, Smuga-Otto K, Howden SE, Diol NR, Propson NE, Wagner R, Lee GO, Antosiewicz-Bourget J, Teng JM, Thomson JA. Nat Methods. 2011 Apr 10.