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The Expi293™ Expression System is a major advance in transient expression technology for rapid and ultra high-yield protein production from mammalian cells. It is based on high density culture of Expi293F™ cells in Expi293™ Expression Medium. Transient expression is powered by the cationic lipid-based ExpiFectamine™ 293 transfection reagent in combination with specialized transfection enhancers. All components work in concert to generate 2- to 10-fold higher protein yields than previous generation transient expression systems such as the FreeStyle™ 293 Expression System. Expression yields of >1 gram per liter of transfected culture have been demonstrated for some antibody and non-antibody proteins.
The Expi293™ Expression System provides:
• Rapid production of milligram to gram quantities of recombinant protein in mammalian cells • High density cultures of Expi293F™ cells that result in more expressing cells per milliliter of culture and higher protein yields • Native folding and mammalian post-translational modifications, such as glycosylation, of expressed proteins • Easy, robust culture and transfection protocols that readily fit into current transient expression workflows • Scalable expression that can be applied to cultures from<1 mL to >10 L, thus generating the amount of protein needed
The components included in the Expi293™ Expression System Kit are: 2 vials of frozen Expi293F™ cells, 1 liter of Expi293™ Expression medium, one ExpiFectamine™ 293 transfection kit sufficient to transfect 1 liter of culture, Opti-MEM® reduced serum media, and an antibody-expressing positive control vector. All components of the Expi293™ Expression System are also available for purchase separately.
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